Principles of Microscopy
Aditya Arya, PhD
Date of publication: 13 Nov 2016
Microscopic techniques are critical to several research domains including biology, Physics and Chemistry and even in clinical diagnosis. Apart from an essential tool in research, microscopes have become a teaching a learning companion for both students and teachers. Since the inventions of first microscope by Hans and Zacharias Janssen in 1590, microscopes have evolved a lot both in terms of improved resolution and magnification. The best microscopes today can have a resolution of less than 1 angstrom and a magnification of over 1 million, while our eyes cannot resolve two dots at a distance lesser than 0.2 mm. Increasing resolution and magnification has created enormous opportunities in research and wider scope of application. Now, microscopes are used in clinical diagnosis, molecular and cell biology studies, nano-material research and even determination of structure of bio molecules.
Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy.
In this Quick note, we will be discussing about basic principles and types of common microscopic techniques and their application in Life Science Research.